Pollen analysis of honey pdf

Pollen analysis of honey and pollen collected by apis. From counts of pollen caught daily, fixed on microscope slides and identified, a graph can be drawn which gives a clear picture of the relative amounts of different species of pollen floating in the air from day to day throughout the growing. It lies in deccan plateau with three major physical divisions viz coast, malnad and maidan. Pollen analyses were performed on honey and beebread from hives in apiaries located in two distinct mangrove areas dominated by laguncularia racemosa l. Absolute concentration is expressed as pollen grains per 10 g of honey and is derived from volumetric data.

Publication date 1943 topics natural sciences, botany, general botany. Melissopalynology is the study of pollen found in honey. Pollen analysis definition is the identification and determination of frequency of pollen grains in peat bogs and other preservative situations as a means of dating fossil and other remains. Altogether, the pollen content of 325 honey samples was analysed with an average of 400. Bees obtain pollen from flowers and by different species 9. For our study, we use a 10g sample of raw honey for the analysis. Moar botany division, dsir private bag, christchurch, new zealand abstract a pollen analytical study of new zealand honey provides a basis for identifying the origins of a honey in terms of locality and floral source. The grains are minute in size, each ranging from 15 to 100 microns. Pollen analysis of honeys from varazdin county, croatia. Pdf an improved method for pollen analysis of honey. A total of 325 samples with a mean of 400 pollen grains each were analysed. Pollen analysis definition of pollen analysis by merriam. Pdf pollen analysis of honey and beebread derived from. Pollen qualitative analysis can provide important data, especially for honey characterization with reference to its geographic origin, oc.

The shannonwiener diversity index h showed that high diversity of plant species was found in eleven honey samples with a range of 1. Pollen analysis of honey it is always useful to know what pollen is in a particular honey and whether it is correct to give it a specific floral origin. Analysis showed that the absence of pollen was consistent regardless of the. Results and discussion the pollen analysis results are shown in tables i and ii and figures 1 and 2. Pollen analysis of honey and pollen collected by apis mellifera linnaeus, 1758 hymenoptera, apidae, in a mixed environment of eucalyptus plantation and native cerrado in southeastern brazil c. Among the nonnectariferous plants, the highest pollen frequency 89. The most common method used to determine the botanical origin of honey is pollen analysis. Pdf filtration, rather than centrifugation, was investigated as a first step to extract pollen from honey. To understand the scale, consider that the head of a pin is about 2 mm 2000 microns in diameter.

In the present investigation, 20 honey samples were collected from apis cerana and apis dorsata colonies during january 2010 to april 2011 from bee hives located at 14 locations of coorg. On the basis of honey pollen analysis, in these 8 samples diff erent botanical origin was determined. The analysis will include extraction of pollen from the honey, pollen concentration values, and a complete analysis report of the recovered pollen data and a list and percentages of the primary pollen and nectar sources. Pdf pollen analysis of honey from the baltic region, estonia. Pdf pollen analysis in honey samples from the two main. Karnataka map chickmagalur map karnataka state extends from 11 0 51 n to 19 0 n latitudes and from 74 0 e to 78 0 e longitudes. Centrifuging should be used but not crushing and straining. When appropriate we will also include probable geographical region where the honey was probably produced. For example, when using pollen counts to determine the nectar sources of a honey sample, we recognize that the types and percen.

In particular, honey sample analyses found from 16 to 41 taxa of pollen grains. Adulteration of honey and available methods for detection a. Pollen analysis in honey samples from the two main. Microscopic analysis and other advanced techniques 11, 8, 12, are currently being used for the analysis of the pollen in honey which may eventually determine its geographical origin. Eight honey samples had lower diversity index values, ranging from 0 arero site 2 and bule hora site 3 to 0. Its scientific scope is broad since it encompasses knowledge from many disciplines including botany, geology, ecology. The national honey board requires beekeepers to have the honey analyzed if they want to label their honey as a monofloral honey. This volume, which will be valuable to both students and researchers alike, is a field and laboratory manual of pollen analysis which provides a wealth of information for the selection of sites, the collection and processing of samples, the identification of pollen covering northern europe and north america, and the analysis and presentation. Biometrician helen nicol, biometrician with nsw agriculture, orange for preliminary analysis of earlier results and analysis. With these filters, a new method based on filtration and followed by acetolysis in microcentrifuge tubes was developed and compared with standard pollen analysis. Th e palynological properties of 8 honey samples commercially produced in 1 region in croatia varazdin county were determined. The samples were submitted to both a qualitative and a quantitative analysis. Palynology is the microscopic study of pollen grains and spores, the fine powdery material produced on the stamens and anthers of seed plants. Pollen analysis is but one of a number of tests that must be used to determine the quality and source of honey.

Quantitative pollen analysis of new zealand honey, poster. Pollen analysis is widely used to verify the geographic origin of honeys, but has never been employed in australia. Honeybee pollen and raw honey are the only food on earth containing all 22 nutrients needed by mankind for complete and perfect health. Honey is a natural and easily digested food produced to the distinctive appearance of pollen grains produced by honey bees. Keywords monofloral honey, pollen, pollen analysis, pollen processing techniques, ethyl alcohol. Honey bee pollen is considered to be a food,and national pollen standards exist in different countries such as brazil,bulgaria,poland and switzerland. Abstract a pollen analytical study of new zealand honey provides a basis for identifying the origins of a honey in terms of locality and floral source. Pollen analysis was carried out on ten honey samples from western ghats, chickmagalur.

Pollen analysis plays a critical role in climate change studies during the quaternary f. To conduct a pollen study of raw honey we first must dilute it before the pollen can be removed for analysis. Melissopalynology, or the pollen analysis of honey, is widely used to verify the claimed geographic and floral origin of honey samples 68. Pollen analysis of honey, or melissopalynology, is of great importance for quality control. The analysis of the pollen content of the honey samples indicates that the local flora may be used as a source of goodquality honey. Honey without pollen here is a list of the honey tested by food safety news that showed no traces of pollen. Figures 1 and 2 allow the visualization of the dominant pollen types occurring in honey samples from the states of ceara and piaui analyzed.

Precision in interpreting pollen data recovered from honey has always been a primary goal of those who study pollen and honey. Pollen analysis of honey, or melissopalynol ogy, is of great importance for quality control. Pdf assessment of honey plant resources through pollen. Characterization of some southern brazilian honey and bee plants through pollen analysis. There are four natural resources required by honey bees for survival.

The sample of raw honey is diluted with 10 ml of distilled water and 150 ml of etoh, and then heated to 100o f to ensure a complete mixture. An improved method for pollen analysis of honey sciencedirect. The present study reports the results of pollen analyses of estonian honey collected from 2000 to 2011. Pollen analysis in honey suhbharani ramnath and sivaram.

The foreword and the authors introduction give some idea of the nature and scope of pollen analysis, the modern phase of which subject began in 1916. According to their standards, 50% of the pollen must be attributed to a single floral source in order to be labeled as a single source. Effects of processing on the pollen and nutrient content of honey. It uses palynological techniques as tools for solving problems in quaternary geology, ecology and archeology. Southern australian vegetation is dominated by eucalyptus myrtaceae forests and, as expected, most australian honeys. Honey pollen analysis honey source verification honey.

Pollen analysis of honey from borana zone of southern. Pure bee pollen bee pollen for human consumtionchemical analysis of honeybee pollen. Analysis of honey chemistry science fair project experiments, chemistry models, exhibition ideas, expo topics for kids and also organics chemistry science ideas for cbse, icse, middleschool, elementary school for 5th, 6th, 7th, 8th, 9th and high school students. Pdf knowledge about the botanical source of honey is very important for the beekeeper while it indicates adequate and abundant supply sources of. The main emphasis was on the floral spectrum of plants and the identification of the most important plant sources for the particular honey. Honey always includes numerous pollen grains mainly from the plant species foraged by honey bees and honeydew elem ents like wax tubes, algae and fungal spores that altogether provide a good fingerprint of th e environment where the honey comes from. Harmonized methods of melissopalynology international honey. Each sample was examined to determine the pollen percentage and pollen spectrum. Review article pollen composition and standardisation of. This spectrum relates to nectarproducing plants, nonnectarproducing plants, contaminations, tainted honey, and mixtures barth 1989. Pdf click to increase image size click to decrease image size.

In this study, we analysed the pollen content of 173 unblended honey samples sourced from most of the commercial honey producing regions in southern australia. This book is a reprint of the fourth edition, published in 1989, of the textbook of pollen analysis and is unique in its approach as it discusses both the practical and theoretical aspects of palynology. Pollen analysis of natural honeys from the central region of. Pollen analysis in honey samples from the two main producing. Nutritional value of bee collected pollens publication no. Here are two methods which a microscopist can use and are based on rex sawyers book honey identification 1988 and my own experiences. The dominant pollen in the state of ceara is from mimosa caesalpiniaefolia. Investigations of pollen analysis of honey samples in india are fragmentary. Micronutrient and pollen contents and antioxidant capacity orac of preblended pb raw honey samples to examine the effects of standard commercial processing i.